Jin Ho Bae

4 Questions 4 Answers 0 Followers

Questions related from Jin Ho Bae

GGGS linker fusion proteins seems to break....?

I am trying to express a fusion protein in BL21 DE3 ecoli strain. [Protein A] - (GGGS)4 - [Protein B] - (GGGS)4 - [Protein C] Above is the construct for the protein. When I express the...

26 March 2019 6,365 3 View

How do you calculate the percentage of ligands bound to receptors given specific kda and concentration?

Lets say A (ligand) binds to B (receptor). I know the kda affinity and the concentration of both. How can I calculate the percentage of % A bound to B at given concentration? Thank you!

11 April 2018 9,241 5 View

Optimal program for designing 3d DNA origami?

Hi I'm trying to design a simple cube-shaped DNA origami with specific dimensions from just single stranded DNA oligos. Since there is no specific scaffold the origami is based on, cadnano is...

17 June 2016 453 3 View

Do silver ions change color in buffer solutions?

I am trying to replicate DNA stabilized silver nanocluster formation and visualize the various fluorescence. However, when I dissolve silver nitrate and mix it just with phosphate buffer (pH 6.7)...

10 February 2015 4,756 10 View