What is the role of KCl in the Second Strand Buffer (Invitrogen)? Can I replace it by the Second Strand Synthesis Reaction Buffer (NEB)?

I want to use RNase H from Invitrogen, but E.coli polymerase I and E.coli ligase from NEB. Can I use the Second Strand Synthesis Reaction Buffer from NEB? Also, NEB states that their System is...

24 November 2015 2,917 2 View