Jillian Porter

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Questions related from Jillian Porter

Is is necessary to use BSA in antibody dilutions if I do a blocking Step for an hour?

My protocol is to block my sample with BSA 1% in PBS-T for an hour at room temp and then add antibodies diluted in BSA 1% in PBS-T. Why do I need to dilute my antibodies in BSA if I do the...

01 March 2022 4,798 3 View

Is RNAse necessary if I'm using DAPI for immunofluorescence??

I'm prepping drosophila ovaries for immunofluorescence. The protocol I'm using uses RNAse A and Propidium iodide for the nuclear stain. My understanding is that if I use DAPI I don't need a RNAse...

01 March 2022 2,795 3 View