Why when I overexpress p53 do I observe a decrease in the amount of chromatin precipitated with an anti-p53 antibody?

I am performing chromatin immpunoprecipitation assays (ChIP) in which I transfect HT-1080 cells with vector control or p53.  When I use an anti-p53, I consistently pull down less DNA in the p53...

19 February 2016 7,740 8 View