Jiivittha Veno

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Questions related from Jiivittha Veno

I need to screen for thermostable lipase which should be active at 50 degree Celsius. How to identify the variants/mutants on tributyrin plate?

As there is halo zone already appeared around the colony which was incubated at 370C on tributyrin plate. The colony of native lipase shows halo zone at 370C too. Please suggest a mutant screening...

02 June 2016 3,825 5 View

How to increase the frequency of mutation by using GeneMorph II Random Mutagenesis Kits (Error prone PCR/directed evolution)?

Is there any conventional methods to introduce mutation in lipase gene to make it thermostable? Any effective protocols for gene cloning and transformation after Error prone PCR?

23 May 2016 7,844 1 View

What is the difference between intracellular and extracellular protein fractions obtained after protein expression?

Cell harvested after protein expression and the fraction (supernatant) obtained is extracellular protein. Next, pellet suspended with buffer and sonicated. Now the supernatant is intracellular...

04 March 2014 8,383 4 View