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Questions related from Jihyun Park
I would like to make cell lines that overexpress using lentivirus. First, 293T cells are transfected, but there is a problem that the virus is not produced well. The target plasmid I use is a...
30 June 2020 6,359 0 View
Vectors and inserts were ligated after cutting with EcoR1 and Xho1, respectively. The colonies were picked and cut again with restriction enzymes. But after cloning, I saw the bands of the...
21 March 2020 665 4 View
I purified the uncut plasmid DNA and performed agarose gel electrophoresis to identify the plasmid. However, no band was seen. The size of the plasmid is about 10 kb, and I loaded it on 0.5%...
25 January 2020 9,510 3 View
I want to clone a target gene into a lentiviral vector to produce a mouse cell line that overexpresses the target gene. I want to know the difference between inserting the full-length cDNA and...
07 November 2019 4,527 2 View
In my experiment, I want to make a cell line expressing target gene + mCherry + luc . (mCherry was selected because there is GFP in the cell line itself, and luciferase will be inserted for in...
25 September 2019 8,509 3 View
