Jifan Yuan

5 Questions 2 Answers 0 Followers

Questions related from Jifan Yuan

How to improve my BrdU immunostaining?

Hi everyone, I am working on BrdU labeling and immunostaining recently. I have tried HCl hydrolysis, it didn’t work so I used 60 degree overnight incubation in citric acid ph6 to denature DNA,...

25 January 2021 595 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

22 January 2021 9,999 3 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

04 January 2021 9,705 3 View

How to reduce the false positives of GST pull-down?

Hi everyone, recently, I am going to study two protein interaction by GST pull-down. I have protein GST-A and MBP-his-B expressed by BL21 through IPTG induction. Initially, I found GST-A can pull...

08 April 2019 2,993 0 View

Is there anybody familiar with cre-ert-gfp mice?

I have been working on this for over one year. After I getting a lot of target cre-ert-gfp mice, I try to localize a group of cells expressing cre-ert-gfp by either immunofluorescence using GFP...

14 October 2017 1,136 2 View