Jichan Gong

3 Questions 7 Answers 0 Followers

Questions related from Jichan Gong

Does the process of deubiquitinaiton need ATP?

i need to perform in vitro ubiquitination assay. now i wonder whether this process need ATP?

03 March 2018 9,243 0 View

How to choose a suitable E2 when performing in vitro Ubiquitination assay?

1. Does the ubiquitination of k48-linkage or k63-linkage share the same E2 in some case ? 2. How to choose a right E2 when identifying whether a protein can act as an E3 ligase for k63-linkage...

08 August 2016 8,646 5 View

Did anybody meet such a problem that half of the 293t died after being transfected meanwhile the color of the medium became very yellow ?

recently when packing lentivirus , my 293t lose attachment to the plate after being transfected and the medium became very very yellow. Even more , the status of target cell after being...

01 January 2016 5,342 6 View