Jibin Sadasivan

4 Questions 5 Answers 0 Followers

Questions related from Jibin Sadasivan

Does anyone have experience of RNA transfection in S2 cells ?

I am trying to express my HA-tagged viral protein in S2 cells. This protein is known to inhibits the cellular transcription. Therefore I tried to transfect with invitro transcribed capped and...

02 February 2017 2,480 3 View

How can I separate/purify 2 KDa peptide after cleaving out GST tag ?

The 2 kDa GST Fusion protein was expressed as a in BL21DE3 expression system. After GST purification I want to remove the GST tag using precision protease and separate out the small peptide. I...

08 August 2015 3,578 4 View

Is it possible express a 180 kDa protein in BL21 DE3 expression system?

I want to raise the antibody against a membrane protein which is of the size 180 kDa. I already have a clone in pET vector. Is there any chance to get a reasonable amount of expression, given the...

09 September 2014 10,000 4 View

What is the importance of tissue glycoforms ?

Same proteins produced in different tissue have different glycosylation pattern.. How is this possible? What is the advantage of adding oligosaccharides to proteins ?

10 October 2012 1,313 3 View