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Questions related from Jiazheng Wang
The band of beta-actin is just like a continuous line. Parameter： Load protein:20ug Run 80V in stack gel 120V in separate gel Wet transfer constant 100V for 1h Use BSA to block for 1h Primary...
02 February 2017 7,209 6 View
this image is from ATCC,so it is absolutey not a contamination. thanks for you help.
06 June 2016 3,719 9 View
It's the first time I culture Caco-2 cells. use 10%FBS DMEM medium，not add antibiotics. culture in 6cm dish. I find cells grow slowly.After 96hours，the confluence is not reach 80% and a lot of...
06 June 2016 1,965 4 View
I use 24mm diameter transwell(0.4μm) to culture Caco-2 cells. I want to detect expression of protein after cells exposed to cytokine. there are three methods first is directly put the lysis...
06 June 2016 5,337 4 View
