Jiaqi Zou

3 Questions 5 Answers 0 Followers

Questions related from Jiaqi Zou

How to fix cells with glutaraldehyde for immunofluorescent staining?

Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...

18 June 2024 3,321 5 View

Immunofluorescence staining for only plasma membrane proteins?

Hello! I am having trouble staining for plasma membrane localization of a transporter. This transporter also resides intracellularly, however, I am only interested in staining its plasma membrane...

29 May 2024 8,216 5 View

Immunofluorescence staining and imaging of endothelial tubules?

Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...

29 May 2024 5,803 4 View