How to isolate normal fibroblast in the colon of C57BL/6 mice?

Hello everyone. Now, I am isolating colonic fibroblasts from C57BL/6 mice, I have tried digestion with 0.05g/ml collagenase type 1, but very few fibroblasts are isolated. Can any research...

13 April 2022 7,692 2 View