Jiabao Tang

2 Questions 4 Answers 0 Followers

Questions related from Jiabao Tang

How to isolation BAC clones from cells?

I am trying to extract Akata BAC from AGS-Bx1 cells and transformed into E. coli DH10B. EBV genes can be detected by qPCR with extacted product. But we cannot picked any EBV-positive DH10B...

12 April 2023 3,872 4 View

Why EBV M81 BAC transfected cells can not be induced into lytic cycle?

I am studing the funtion of an EBV encoding protein. M81-BAC was transfected into AGS, HK1 or HEK293t cells using lipofectamine 3000 and cultured with hygromycin B. GFP-positive cell colonies...

12 April 2023 1,692 5 View