What is the suitable culture condition for culturing cryopreserved PBMCs for 2-3days to obtain maximal viability rate using flow cytometry?

I have been trying to culture cryopreserved PBMCs, but PBMCs with just media alone (after 48 hours) were giving me very low viability rate when acquired on a flow cytometry. The viability rate...

11 May 2015 6,461 18 View