How do I shorten the time to get HUVEC align under shear stress?

I have tried 30-90% confluent HUVEC under 15 or 20dyn/cm2. In all cases, cells need 72h to get alignment.  I use ibidi system. HUVEC were cultured in medium containing 20% FCS.  What conditions...

05 November 2015 2,149 6 View