Jewel Ann Joseph

3 Questions 5 Answers 0 Followers

Questions related from Jewel Ann Joseph

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

08 February 2021 4,081 9 View

Which is the best buffer solution to resuspend protein pellet prior to HPLC analysis?

I am working with a sweet-protein 'Thaumatin' (pKa ~ 11) which is secreted by genetically modified Pichia pastoris GS115. Since the protein-producing organism is grown in Basal salts medium, the...

27 October 2020 5,693 3 View

Does anyone know a simple and inexpensive lab-scale purification step for biological samples?

Hi, I am currently producing a recombinant protein from yeast Pichia pastoris. The medium used in the bioreactor is the Basal salt medium supplemented with PTM1 salts. I have noticed that when...

20 October 2019 8,554 5 View