Jessica Feyen

2 Questions 3 Answers 0 Followers

Questions related from Jessica Feyen

Can I use 0.5 mm glass beads for cell disruption of E. coli?

I have previously been using a high pressure cell disruptor, but it's currently unusable until we get a replacement part. In the mean time, I'm trying to purify an enzyme out of E. coli and last...

09 September 2015 9,018 8 View

Does the order of steps in purification of His and FLAG tagged dimer matter?

I'm purifying a homodimer that is coexpressing subunits with different tags to be able to mutate subunits independently from eachother. One subunit has a His tag and the other a FLAG tag. My...

06 June 2015 6,828 4 View