Has anyone had problems with a lentiviral system and EcoR1 restriction site and getting corresponding GFP expression?

Hello:  We are using a lentiviral plasmid - pLVx-EFalpha1-AcGFP-N1 and we cloned a very large protein (about 9000bp) and a smaller protein (about 500bp) into the plasmid. We used EcoR1 for the...

04 March 2015 8,343 4 View