I am losing almost 50% yield in my RNA EtOH precipitations. Suggestions?

Here is my typical protocol: 1) To 200 ul of ~1.2 nmol of 95 bp RNA, I add... 1 ul 20 mg / ml glycogen as a carrier.3 M final concentration sodium acetate, pH 5.2 at room temp.01 M final...

21 July 2015 4,831 7 View