Why is there nuclear debris when I transfect HeLa cells and stain with Hoechst?

I transfected HeLa cells with 0.3uL of XtremeGene-9 and 100ng of GFP-tagged DNA. After two days I fixed the cells with 4% Formaldehyde in PBS for ten minutes, then washed with 0.1% Triton X-100 in...

13 June 2016 6,137 6 View