Jessica Lauren Davis

2 Questions 4 Answers 0 Followers

Questions related from Jessica Lauren Davis

Vented Caps or No Vented caps on Spinner Flask For Cerebral Organoids?

Hi all, I am wondering if anyone knows whether to use vented caps on the spinning bioreactor or non-vented caps. I am following the Lancaster protocol but it does not specify whether the caps are...

17 December 2018 8,155 1 View

Has anyone managed to get good protein yield from organoids?

I have tried to lyse organoids for protein extraction using RIPA with protease and phospatase inhibitors. I found that from 5 pooled organoids my yield was low (1-2ug/ul). Has anyone tried another...

20 December 2017 4,907 4 View