Jesse Jones

2 Questions 31 Answers 0 Followers

Questions related from Jesse Jones

How do I take the ‘intrinsic activity’ of an enzyme into account when trying to fit the data to an appropriate equation (if I need to at all)?

I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase...

01 January 2019 8,224 5 View

What is the easiest way to fix a single nucleotide missense mutation in a gene insert on a plasmid?

I've cloned the gene for a target protein into a plasmid and, upon sequence verification, caught a single nucleotide missense gene mutation (L to P). Does anybody have a favorite way of dealing...

09 September 2016 741 6 View