Jess Krieger

4 Questions 2 Answers 0 Followers

Questions related from Jess Krieger

How much sequence consensus do I need between homology arms and genome sequence for HDR?

I received a plasmid with two homology arms (5' = 510bp and 3' = 1012bp) that I would like to use for homologous recombination with the Cas9 enzyme. I BLASTed the sequence of my homology arms to...

11 July 2019 2,589 0 View

Anyone know of a good CD34 antibody for bovine aortic endothelial cells?

I'm culturing bovine aortic endothelial cells and will soon take them into co-culture. I need to distinguish these cells from other cell types, so I'm thinking the CD34 antibody will be useful....

30 October 2017 4,558 2 View

How to prevent fibrin hydrogel collapse?

I recently started working with fibrin hydrogels. I'm using bovine aortic endothelial cells and embedding them in a fibrinogen (10mg/mL) / thrombin (1.25U/mL) mixture. The hydrogels seem solid and...

20 September 2017 7,869 6 View

How do you image GFP+ cells through a gelatin-based hydrogel?

I'm going to embed GFP+ cells into a gelatin hydrogel, and I'd like to take live and dead images of them. I've seen a few protocols on how to image through hydrogels, but I'm not sure how...

20 May 2016 920 3 View