Jess Kopenhaver

3 Questions 2 Answers 0 Followers

Questions related from Jess Kopenhaver

Help with passaging viable PDX spheroids?

I've been trying to passage my PDX spheroids that are approximately 100-250 um in size. I plate ~25K cells/well after tumor dissociation with collagenase II/ DNAseI and magnetic separation with...

19 August 2021 796 0 View

Does anyone know how to perform Consensus Molecular Subtyping for CRC?

I am attempting to perform Consensus Molecular Subtyping (CMS) on colorectal cancer specimens that my lab has collected over the years. I am struggling to find a method that is streamlined, and...

19 August 2021 2,394 1 View

Can you remove a secondary antibody from mounted immunofluorescent-stained tissue?

I used an Alexa 488 anti-rabbit secondary antibody for colon tissue samples (that had gone through FFPE and rehydrated, followed by antigen retrieval) but the stain was really weak and the signal...

14 July 2021 3,783 2 View