Jeslin Varghese

2 Questions 2 Answers 0 Followers

Questions related from Jeslin Varghese

What may be the possible reasons for getting a Ct value in case of non template control during qRT PCR?

In my lab we carried out qRT PCR for checking the efficiency of the primers. The results came fine for dilutions, unfortunately for non template control also we got good expressions. Inorder to...

15 January 2021 9,694 5 View

I have an issue in bacteriophage propagation. Why the host bacteria is coming as separated colonies without any lysis?

I am propagating bacteriophage phi x 174, with its host bacteria E.coli C strain. I prepared E.coli C strain broth culture of OD600 0.4. Then I took 100 micro litres of E.coli and added to soft...

19 October 2020 5,438 4 View