Jerome Moriniere

4 Questions 9 Answers 0 Followers

Questions related from Jerome Moriniere

I am looking for new ways to circumvent effects of cross-talk in metabarcoding samples (CO1, 16s, ITS2) - anyone has experience with that?

When analyzing hundreds of metabarcoding samples using NGS primers (e.g. CO1, ITS2, 16s) some samples show clear effects of cross-talk - is there a way to circumvent these phenomenons by using...

01 January 2018 6,225 10 View

Does anybody have experience in establishing a CO1 reference library in Geneious?

How can I establish my own reference library for NGS data? Does somebody know an easy way to establish a tool to BLAST or compare hundreds of thousands of CO1 sequences against a reference library...

10 October 2014 9,882 3 View

Why use M13F tailed primers for PCR and sequencing? What causes the real benefit in contrast to the usual primer?

Has anyone some experience with the use of M13F tailed primers?

09 September 2013 1,581 13 View

Problems with amplification of COI (Hco/Lco)

Anybody have experience with problems with the amplification of Hco/Lco after extraction of fresh specimen from mallaise trap samples? While the amplification of COI (old samples work, so its not...

03 March 2013 1,763 8 View