Jeremy Mullesa

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Questions related from Jeremy Mullesa

What plasmid type that you get from isolation of a culture?

Greetings for all of scientist using this platform. I have a little problem. Recently I had done reconstruction of my plasmid (Named PDR111, length = 11,8 kb). Transformed culture i named it W1...

22 April 2024 7,789 4 View

How to confirm your ligation before its transformation in E.coli?

So let say I have circular plasmid named "PDR111". I did PCR with KOD ONE MIX KIT and the product is linear DNA. Then, I did self circularization of linear DNA with T4 DNA Ligase protocol (Thermo...

12 March 2024 1,113 7 View