Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

09 March 2020 1,126 3 View