Jeremy Linsley

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Questions related from Jeremy Linsley

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

09 March 2020 1,169 3 View

When trying fluorescent in situ hybridization of the drosophila larvae brain, why does the vasculature non-specifically stain?

I am trying to label extracted drosophila larvae brains by fluorescent in situ hybridization with fast red. Our lab has previously done plenty of in situ hybridizations on zebrafish embryos with...

27 August 2014 9,000 1 View