Jérémy Augustin

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Questions related from Jérémy Augustin

Can restriction enzyme integrate cytosol when transfecting cells with lipofection for establishing stable cell lines?

Hello, I Wonder why we have to heat inactivate restriction enzyme before transfecting cells with a linearized plasmid with Lipofectamine (3000) to establish stable cell lines. What if my enzyme is...

11 August 2016 6,992 4 View

Can restriction enzyme integrate cytosol when transfecting cells with lipofection for establishing stable cell lines?

Hello, I Wonder why we have to heat inactivate restriction enzyme before transfecting cells with a linearized plasmid with Lipofectamine (3000) to establish stable cell lines. What if my enzyme is...

11 August 2016 9,989 2 View