Jeremías Gastón Galletti

3 Questions 11 Answers 0 Followers

Questions related from Jeremías Gastón Galletti

Why am I getting such different kinetics in an allogeneic T cell stimulation assay?

I'm evaluating the capacity of some epithelial cell supernatants to induce dendritic cell maturation. I take murine bone-marrow-derived dendritic cells on day 6 (DCs) and culture them for 48 h in...

09 September 2013 7,710 8 View

What is the optimal ovalbumin dose for a footpad delayed-type hypersensitivity assay in mice?

I´m performing DTH assays in mice. I usually immunize with 100 ug OVA in complete Freund´s adjuvant (1:1 mixture) by a subcutaneous injection, wait for two weeks and then inject 100 ug...

09 September 2013 3,305 5 View

How can I prepare heat-aggregated ovalbumin that is suitable for footpad injection in mice?

I have grade V ovalbumin. I have tried to heat a 20 mg/ml solution at 60 C for 10 min and I ended up with coagulated protein, almost like cooked egg white. Does anyone know of a good protocol for...

08 August 2013 290 1 View