Jeremías Gastón Galletti

3 Questions 5 Answers 0 Followers

Questions related from Jeremías Gastón Galletti

What is the expected efficiency of T cell transfer in mice?

Which percentage of labeled T cells should I find in the peripheral lymph nodes after adoptive transfer of say 5-10 million labeled T cells? Is there a difference in efficiency between the...

16 June 2017 740 1 View

What is the optimal ovalbumin dose for a footpad delayed-type hypersensitivity assay in mice?

I´m performing DTH assays in mice. I usually immunize with 100 ug OVA in complete Freund´s adjuvant (1:1 mixture) by a subcutaneous injection, wait for two weeks and then inject 100 ug...

04 September 2013 1,613 4 View

How can I prepare heat-aggregated ovalbumin that is suitable for footpad injection in mice?

I have grade V ovalbumin. I have tried to heat a 20 mg/ml solution at 60 C for 10 min and I ended up with coagulated protein, almost like cooked egg white. Does anyone know of a good protocol for...

20 August 2013 8,989 1 View