Jenny Yang

2 Questions 6 Answers 0 Followers

Questions related from Jenny Yang

Will urea do harm to the HPLC column?

Usually I use TFA to dissolve my peptide (Abeta40) for HPLC purification with C18 or C8 column. Recently I tried to use urea to dissolve the peptides before injection, but after that, the...

03 March 2015 659 5 View

Is centrifugation necessary when using thin-film method to prepare liposomes?

I use thin-film method with 20 mM soy PC (Avanti) and 6 mM cholesterol hydrating in PBS to make liposomes, in order to encapsulate peptides (40 residues). My procedure is as followed: 1. prepare...

03 March 2014 256 9 View