A FISH protocol (DNA beacons for Epifluor) says denaturation @ “150 deg C”. Is this too hot?

I have 2 protocols for FISH w/ DNA beacons (approximately 25 nucleotides long) for use on formalin-fixed paraffin embedded tissue (5µm samples) for examination with epifluorescence. One protocol...

01 January 2016 9,788 3 View