Jennifer Kate Demuro

3 Questions 5 Answers 0 Followers

Questions related from Jennifer Kate Demuro

Does anyone have experience using soy milk as a blocking agent for IR western blotting?

We recently started using a Licor Odyssey Fc machine and got great results with the blocking buffer that Licor provides with the machine. However, we have used almost all of it and it is really...

11 November 2014 8,278 9 View

How do I concentrate a protein sample (has too much lysis buffer)?

I am performing western blots on some old brain tissue and I did a BCA and I basically would need to add 200ul of each protein sample to achieve the right concentration which obviously too much...

01 January 2014 5,859 4 View

Question on western blot: should we block before adding another antibody of interest?

Lets say I have an antibody for HDAC5 which I will visualize, then incubate with GAPDH and visualize. After this step I would wash 2x in PBS and then could I add my next antibody of interest (TNF...

11 November 2013 6,694 15 View