Jenna Ridsdale

3 Questions 11 Answers 0 Followers

Questions related from Jenna Ridsdale

What are the most likely reasons for low efficiency of a co-immunoprecipitation?

I have recently been trying to optimize co-immunoprecipitation in K562 cells. I have tried 6 different antibodies for my protein of interest (all compatible with IP) and am using Protein G-PLUS...

03 March 2015 1,694 14 View

What considerations need to be taken into account when choosing a plasmid construct?

I've just started my PhD and am wanting to search for a construct on Addgene that expresses a tagged version of my protein of interest. It's really just a preliminary search to see what constructs...

10 October 2013 2,752 2 View

Could anyone advise me on Flag tag IP-MS?

I've been reading a lot of literature on tagging proteins for analysis in MS and am getting a bit confused by one aspect. I'm wondering what the consensus view is as to which beads are the best...

10 October 2013 4,703 2 View