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Questions related from Jen i-chen
Thanks in advance for your replies.
13 May 2015 9,681 1 View
I transformed four different constructs into agro seperately (one of them is p19, silencing surpressor). Mix all four kinds of agro cultures with equal volume. (final OD=1) Inject the mixed...
12 May 2015 2,543 1 View
What is the purpose of input? Before I perform coip, I take some sample for input control. But input should be analyzed by PAGE on same gel with sample after coip. During this time period (when...
04 May 2015 5,990 7 View
In the coip experiment, substrate band can only be enriched by e3 ligase in the presence of MG132. So when there is no MG132, once the substrate binds to e3 ligase, ubiquitylation occurs at a very...
04 May 2015 9,768 1 View
I read a paper. The authors performed in vivo coimmunoprecipitation to investigate the interaction between rsl1 (e3 ligase) and ABA receptor (putative substrate protein). ABA receptor band can be...
30 April 2015 9,712 2 View
In BiFC, we always coexpress two proteins of interest in one cell to investigate the interaction. And we usually use the Agrobacterium to infiltrate the tobacco cells. How can researchers select...
26 March 2015 3,409 3 View
I found a paper on the plant journal. In BiFC, the authors fuse YFPN to C-terminal of a plasma membrane protein called RSL1 (at the outside of the cell). And they fuse YFPC to a soluble protein...
18 March 2015 5,964 4 View
