Jen i-chen

11 Questions 2 Answers 0 Followers

Questions related from Jen i-chen

Can Brefeildin A inhibit transportation or maturation of early endosome to MVB in plant cells?

BFA compartment is composed of TGN/EE in plant cells. And it is composed of early endosome and endocytic vesicles in animal cell. Am I right? If this is true, it means BFA can inhibit downstream...

22 May 2015 6,722 2 View

Do you have any review about regulation mechanism of plant E3 ligases, especially for RING and HECT type?

Thanks in advance for your replies.

13 May 2015 9,700 1 View

How can I transient co express two or more proteins in tobacco leaves through agrobacteria?

I transformed four different constructs into agro seperately (one of them is p19, silencing surpressor). Mix all four kinds of agro cultures with equal volume. (final OD=1) Inject the mixed...

12 May 2015 2,565 1 View

How do I prepare input control for coimmunoprecipitation?

What is the purpose of input? Before I perform coip, I take some sample for input control. But input should be analyzed by PAGE on same gel with sample after coip. During this time period (when...

04 May 2015 6,012 7 View

Is the interaction between E3 ligase and substrate transient?

In the coip experiment, substrate band can only be enriched by e3 ligase in the presence of MG132. So when there is no MG132, once the substrate binds to e3 ligase, ubiquitylation occurs at a very...

04 May 2015 9,791 1 View

How can I compare the results of coimmunoprecipitation between e3 ligase and its substrate with or without MG132?

If I performed coip between e3 ligase and its putative substrate with or without MG132, the substrate band would be enriched by e3 ligase with MG132. So I say that MG132 stabilizes the protein...

04 May 2015 3,715 3 View

Will e3 ligase-substrate interaction be affected by MG132 treatment during coip?

I read a paper. The authors performed in vivo coimmunoprecipitation to investigate the interaction between rsl1 (e3 ligase) and ABA receptor (putative substrate protein). ABA receptor band can be...

30 April 2015 9,731 2 View

Why can MG132 inhibit ubiquitination?

MG132 targets proteasome to inhibit its activity, but it does not inhibit E3 ligase. Then why will MG132 will lead to accumulation of unubiquitylated protein level in a cell?

30 April 2015 6,148 4 View

How do I coimmunprecipitate an e3 ub ligase and its substrate?

To investigate the interaction between e3 ligase and its substrate, in vivo coip is conducted. If we want to see the interaction, we have to add MG132 during coip. If we didn't add MG132, we could...

29 April 2015 3,983 4 View

How do I control transient coexpress of different proteins in infiltrated tobacco leaf cells?

In BiFC, we always coexpress two proteins of interest in one cell to investigate the interaction. And we usually use the Agrobacterium to infiltrate the tobacco cells. How can researchers select...

26 March 2015 3,431 3 View

How can YFPN and YFPC combine in different cell compartments?

I found a paper on the plant journal. In BiFC, the authors fuse YFPN to C-terminal of a plasma membrane protein called RSL1 (at the outside of the cell). And they fuse YFPC to a soluble protein...

18 March 2015 6,017 4 View