Different concentration of HRP causes the same color on TMB. Why?

Hello everyone~ I prepared 1-20 nM of HRP (Mw= 40k) and then added 20 uM of TMB (sigma tablet) and H2O2. Finally, I got the exact same color (under uv-vis) after 1-30 mins. All solutions were in...

18 April 2022 6,992 0 View