Is it possible to phenotype (stimulated) Tregs in PMA/I stimulated PBMCs culture and detect their expression of IL-10 by flow cytometry?

I am trying to detect stimulated Tregs and their IL-10 expression in cultured PBMCs (not isolated from the cells mixture) by flow cytometry. For other T cells phenotyping and evaluating of...

06 June 2015 9,935 10 View