Jeffrey Lakritz

4 Questions 47 Answers 0 Followers

Questions related from Jeffrey Lakritz

When evaluating performance of ELISA assay on a particular day or between days how does the group evaluate accuracy and precision?

I have been asked to provide within-day and between day CV% for data produced using ELISA. While the negative and positive controls appear to perform as they should, the absorbance values for the...

08 August 2018 1,815 0 View

What does the shape of the attached standard curve mean?

We are using a variety of ELISA assays in our lab. With one in particular, we appear to be having issues with a narrow range of concentrations that can be used. This appears to require dilution...

12 December 2012 7,698 35 View

How to isolate bone marrow progenitor cells?

I am interested in gradient centrifugation of bone marrow cells that I could use to study gene and protein expression. I want to study neutrophil development in livestock. I understand this is...

12 December 2012 1,886 6 View

Simultaneous expression of 2 different proteins from the same cell line. What is the likelihood both are expressed?

Expression of two different recombinant proteins from optimized DNA sequences.

10 October 2012 5,305 13 View