How can I express a protein from a cDNA in pANT7-cGST vector, using a mammalian in vitro expression system ?

I have an already-cloned cDNA which encodes a human protein. It is cloned in the pANT7-cGST vector (map: http://dnasu.org/DNASU/GetVectorDetail.do?vectorid=157). Has anybody a protocol (and...

06 June 2015 981 3 View