Jean Neil

3 Questions 1 Answers 0 Followers

Questions related from Jean Neil

How to set up the analysis for live/dead cells on facs?

I have isolated spleen cells and i want to check if i have live or dead cells. I have stained them with live dead aqua from invitrogen. I am using BD DIVA for facs. Which diagrams should i draw...

19 February 2018 6,130 0 View

Why do i have late amplification of my gene in qPCR even if i add more cDNA

I have done RNA isolation from a kidney cell line ( RIN 9.3), followed by reverse transcription using 1ug of RNA using iscript kit. But after qPCR, I found the positive control Ct value is 29...

26 July 2017 1,095 6 View

How to calculate cDNA concentration after reverse transcription ?

We had the following A human blood sample had RNA concentration of 142,85 ng/ul, we used 3,43 ul in 20 ul (with a mass of 490 ng) using the iscript kit. Nanodrop reading for this blood sample...

12 July 2017 9,264 3 View