Jean Etersque

2 Questions 6 Answers 0 Followers

Questions related from Jean Etersque

Constitutively-active RhoA in HEK293T cells; Need microscopy help; do these cells look positive?

I am developing stably transduced HEK293T cells (lentivirus) with constitutively active RhoA gtpase. I have no experience in Microscopy and I want to characterize these cells visually and expand...

16 February 2022 4,264 4 View

Anyone have a good PBMC Cytokine Protocol? How long do you incubate PBMCs with peptides? and do you need to spike the doses over time?

Need to detect cytokines from PBMCs dosed with peptides. This is to determine if the peptides elicit PBMCs to release markers for immune response. I am unsure how long to incubate the PBMCs with...

03 December 2020 4,827 6 View