Jazib Ali Irfan

3 Questions 6 Answers 0 Followers

Questions related from Jazib Ali Irfan

Trying to regenerate Nicotiana benthamiana transformed with Chloroplast Transit Peptide + aadA gene but still unsuccessful. Why?

I have transformed nucleus of Nicotiana benthamiana with LBA4404 Agrobacterium strain harboring gene of interest that was Chloroplast Transit Peptide + aadA gene for spectinomycin resistance. I...

20 May 2018 2,113 6 View

Does GFP ligated in plant vector expresses in Agrobacterium LBA4404 strain?

I transformed agrobacteirum (LBA4404) cells with pGREEN-GFP vector but the GFP doesn't seems to be expressing in the LBA4404 cells, what is the problem? Why I am not getting any GFP expression? Is...

30 January 2018 7,039 4 View

Not getting colonies of E.coli transformed with ligation. Why?

I eluted backbone from pGHC-GFP vector and insert from CryIIAb after restriction with NdeI and XbaI and ligated them for 1 hour with T4 DNA ligase at room temperature for 1 hour, then I...

02 November 2017 3,559 5 View