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Questions related from Jazib Ali Irfan
I have transformed nucleus of Nicotiana benthamiana with LBA4404 Agrobacterium strain harboring gene of interest that was Chloroplast Transit Peptide + aadA gene for spectinomycin resistance. I...
20 May 2018 2,113 6 View
I transformed agrobacteirum (LBA4404) cells with pGREEN-GFP vector but the GFP doesn't seems to be expressing in the LBA4404 cells, what is the problem? Why I am not getting any GFP expression? Is...
30 January 2018 7,039 4 View
I eluted backbone from pGHC-GFP vector and insert from CryIIAb after restriction with NdeI and XbaI and ligated them for 1 hour with T4 DNA ligase at room temperature for 1 hour, then I...
02 November 2017 3,559 5 View