Jayesh Puthumana

4 Questions 7 Answers 0 Followers

Questions related from Jayesh Puthumana

Is it possible to make mutation in stopcodon at the end of an ORF by simply changing stopcodon bases in the reverse primer?

In a case, one ORF with a TAA stopcodon at its 3’ end. Can I modify the base sequence (eg instead of TAA, I want to change as TTA to modify the stopcodon by incorporating the mutated base sequence...

09 September 2014 3,162 7 View

Is it possible to express a GFP tagged protein without the help of a vector (along with a promoter)?

see above

03 March 2014 4,083 6 View

How can I tag a fluorochrome with Beta glucan?

Can I tag Aniline blue with glucan? Is it possible to identify the presence of glucan in the cytoplasm of a eukaryotic/yeast cell by using aniline blue or any other fluorochrome?

03 March 2014 1,418 0 View

How to differentiate cells in vitro under "nutritional deficiency" (stress) based on cytoskeleton (actin filaments) structure ?

Is it possible to identify the stress fiber formation using phalloidin conjugated fluorescent dye ?

03 March 2014 6,310 6 View