Jayesh Puthumana

4 Questions 5 Answers 0 Followers

Questions related from Jayesh Puthumana

How can I analyse the Community Structure using 16S RNA gene sequencing?

Can anybody help me to do the community structure analysis using 16S RNA gene probes. Is there any reliable method other than NSG? Is there any software to analysis the community structure/ abundance?

28 January 2015 3,046 3 View

How do I separate biofilm from a plastic bead?

Which methods/chemicals can be use to separate the biofilm from a plastic bead. Tried many steps to extract complete DNA from biofilm attached to plastic beads used in a nitrifying bioreactor....

01 November 2014 223 4 View

Is it possible to make mutation in stopcodon at the end of an ORF by simply changing stopcodon bases in the reverse primer?

In a case, one ORF with a TAA stopcodon at its 3’ end. Can I modify the base sequence (eg instead of TAA, I want to change as TTA to modify the stopcodon by incorporating the mutated base sequence...

14 September 2014 6,181 6 View

How can I tag a fluorochrome with Beta glucan?

Can I tag Aniline blue with glucan? Is it possible to identify the presence of glucan in the cytoplasm of a eukaryotic/yeast cell by using aniline blue or any other fluorochrome?

13 March 2014 4,413 0 View