Jayedul Hassan

4 Questions 14 Answers 0 Followers

Questions related from Jayedul Hassan

Is there any protocol to isolate a particular plasmid from a bacteria containing 2 or more plasmids?

I have a bacteria and upon SI nuclease PFGE I came to know that it contains 2 plasmids (140 k and 350 kb respectively). I am interested to isolate one of the plasmids. Once I tried to isolate the...

19 March 2016 9,141 6 View

What is the protocol for doing a Vero cell cytotoxicity assay for the determination of anti-virulence effect of a compound?

Hi there, Actually, I am very new in cell cytotoxicity assay. I am planning to check the anti-virulence activity of a compound (i.e. whether this compound can inhibit the production of...

08 April 2015 7,643 0 View

Does anybody have anti-stx1 and/or anti-stx2 antibodies?

I have some E. coli isolates who are positive to stx1 and stx2 genes by PCR. Now I am interested to know whether my isolates are producing stx1 and stx2 toxins and quantify them. In my lab we have...

05 December 2014 10,034 3 View

Culturally positive but negative in PCR!!!

I have been isolating 120 E. coli from rectal swabs of cattle to confirm whether there is any O157:H7. I have cultured them onto a selective media - Sorbitol MacConkey agar with Cefixime...

23 June 2013 5,657 4 View