Jaya Sharma

3 Questions 2 Answers 0 Followers

Questions related from Jaya Sharma

Why is the GST tag getting cleaved during protein expression?

I am trying to express a small protein, i.e, 12 kDa with an N-terminal GST tag. I have tried various post induction time durations as well as incubation temperatures. Though I see the expression...

07 October 2019 8,247 1 View

Protocol for quantification of phosphatase enzyme in bacterial protein samples using pNPP?

I need to quantify the phosphatase enzyme present in the bacterial protein samples.. I shall use p-nitrophenyl phosphate as the substrate. I need to know the methodology to carry out the...

20 September 2016 1,969 1 View

Why does the PCR product degrade soon?

I have been using the jumpstart red taq polymerase mix from Sigma. I get a good amplification but the problem arising is that it starts degrading within 48 to 72 hours. Next time I'll purify the...

24 June 2014 9,499 3 View