Jatin Sharma

4 Questions 3 Answers 0 Followers

Questions related from Jatin Sharma

RMSD plot doesn't converge?

I am trying to simulate a protein bound to a ligand but I can't see the point of convergence in the RMSD plot. The protein I am using has some chains missing. Could that be the cause?

08 August 2016 4,378 2 View

After membrane transfer of proteins in Western blot, Will the proteins remain on the membrane if I were to rub the membrane with my hands?

The question is about how strongly the proteins bind to the membrane. Is the rubbing of my hand going to significantly affect the retention of the proteins on the membrane? Not that I will be...

06 June 2016 3,124 3 View

My TBST buffer shows some cloudiness after the addition of primary antibodies to the Nitrocellulose membrane. Can it be a fungal growth?

I am performing a Western blot for the NLR adapter ASC protein but during the primary antibody addition, there is some turbidity in the buffer. I am sure this is not a precipitation reaction. What...

06 June 2016 1,727 3 View

Which tool is used to draw cell signalling pathways in windows?

It would be very helpful to know.

11 November 2015 566 0 View