Jason A Metcalf

4 Questions 10 Answers 0 Followers

Questions related from Jason A Metcalf

What level of sequence similarity is required for analyses of positive selection?

Hi folks, I am comparing two rather divergent homologs of the same protein-coding gene and am interested in whether there has been any regional variation in selection along its coding sequence. I...

07 July 2014 5,882 3 View

Weird results with high percentage PAGE gel?

I'm trying to express and purify a small, 6 kDa protein in E. coli and was told to detect expression I needed to run a 20% PAGE Gel. I've never used anything that thick before, and most often use...

02 February 2014 5,693 18 View

Anyone seen qPCR curves like this?

So I got these weird qPCR amplification curves today using cDNA from archaea cell culture. I extracted RNA using Trizol and performed a DNase digestion on 100ng of RNA to remove any contaminating...

05 May 2013 2,006 44 View

What's a good control gene for qPCR in Archaea?

I know GAPDH, beta-actin, tubulin, etc. for mice, but I want to study the expression of a gene in a thermoacidophilic archaea species. I can't seem to find papers anywhere of people doing this...

03 March 2013 3,070 14 View