Jason Shirian

2 Questions 8 Answers 0 Followers

Questions related from Jason Shirian

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

28 January 2021 9,063 3 View

What may be causing loss of nickel from nickel beads when purifying proteins from p. pastoris medium?

For those not familiar with expression with p. pastoris yeast: proteins are secreted into the growth medium. This is the medium: 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0,...

09 January 2020 3,377 4 View